The dense microbial communities inhabiting Lost City chimneys are the most dramatic examples of life powered by the geochemical reactions known as serpentinization. Ongoing work in the Brazelton Lab includes analyses of metagenomic data recovered from Lost City chimney samples.

 

A new expedition to return to the Lost City has been funded by NSF and is currently scheduled to set sail in September 2018. Stay tuned!

 

Lost City images and geological data are available at the Lost City website, hosted by the University of Washington.

 

Further Reading

 

Reports from previous Lost City Expeditions:

Daily Update, 2008 Mid-Atlantic Ridge Cruise

Intro to Lost City Microbiology, NOAA Lost City Expedition 2005

Expedition Log, 2003 Lost City Cruise

 

News articles about Lost City:

http://www.npr.org/templates/story/story.php?storyId=123447937&ps=cprs http://www.sciencedaily.com/releases/2010/01/100111155108.htm

http://www.treehugger.com/files/2010/01/winning-genetic-diversity-race-lost-city.php German public radio story translated into English

 

Public Data

 

These are files useful for analyzing the JGI assembly of metagenomic sequences from a Lost City chimney:

Artemis file for visualizing contigs and best BLAST hits. You can download Artemis here.

LostCity_chimney_metagenomic_contigs.artemis

 

Files generated by the Joint Genome Institute containing information about paired end sequences and contigs:

combined.4082501.4082545.lucy.pga.assem.readpos

combined.4082501.4082545.lucy.pga.assem.contigs.link

combined.4082501.4082545.lucy.pga.assem.contigs.info

 

This FASTA file contains all 6324 contigs in the assembly (zip compressed):

LostCity_combined_assembly.fasta

 

This FASTA file contains all 46,360 sequencing reads used to make the assembly (zip compressed):

combined_reads.noN.fasta

 

This folder contains all transposase-related PFAM seeds used in the transposase BLAST search described in the paper cited below (zip compressed):

integrase_transpo_combined.zip

 

References:

Brazelton WJ, Baross JA (2009) Abundant transposases encoded by the metagenome of a hydrothermal chimney biofilm. ISME J 3: 1420-1424. [PDF]

 

Brazelton WJ, Baross  JA (2010)  Metagenomic comparison of two Thiomicrospira species inhabiting contrasting deep sea hydrothermal environments PLoS ONE. [HTML]

 

Brazelton, W.J., B. Nelson and M.O. Schrenk (2012) Metagenomic evidence for H2 oxidation and H2 production by serpentinite-hosted subsurface microbial communities. Frontiers in Extreme Microbiology. 2:268. doi: 10.3389/fmicb.2011.00268. [PDF][HTML]